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Bone
Marrow
Description
Includes short term (usually 24 and 48 hour) unstimulated cultures
of bone marrow cells. For suspected chronic B-cell malignancies,
longer term (3-7days) pokeweed stimulated cultures are established.
Cytogenetic evaluation includes detailed analysis of 20 cells and
at least two banded karyotypes. The referring physician is called
with results as soon as they are available.
Indications
• suspected hematologic malignancy
• chronic myelogenous leukemia and other myeloproliferative
disorders
• myelodysplastic syndrome
• acute myelogenous leukemia
• acute lymphocytic leukemia
• chronic lymphocytic leukemia and other chronic lymphoproliferative
disorders
• lymphoma
• follow-up after chemotherapy, radiation, or bone marrow transplantation
• rapid determination of karyotype of newborn with multiple
severe congenital anomalies
Specimen Requirements
2 cc bone marrow in a green-top (sodium heparin) tube or 2 cc bone
marrow with 0.3 cc sodium heparin in a red-top tube. Invert several
times to mix bone marrow. Culture of clotted marrow often does not
result in spontaneously dividing cells necessary for cytogenetic
analysis. Other anticoagulants such as lithium heparin may be harmful
to the viability of the cells. Specimen may be refrigerated. Specimen
cannot be frozen. Label tube with patient's name and laboratory
control number. Send to Great Lakes Genetics as soon as possible.
Include a copy of a recent peripheral blood CBC with differential
and indicate the suspected diagnosis on the Great Lakes Genetics
Requisition Form.
Reference Value
46,XX or 46,XY
Turn Around Time
2-8 days (generally 3 days)
CPT Codes
88262 - chromosome analysis, hematologic disorders
88237 - chromosome analysis, tissue culture for bone marrow
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